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CH Instruments cona polysaccharide

Effect <t>of</t> <t>chitosan</t> on <t>ConA‐induced</t> T‐cell proliferation in vitro. Mesenteric lymph node (MLN) lymphocytes from normal rats labelled with succinimidyl ester of carboxyfluorescein diacetate (CFSE) were stimulated with 2.5 μg/ml of mitogen in presence or absence of chitosan (1–100 μg/ml) at 37 °C in RPMI 1640 medium supplemented with 10% foetal calf serum in a 95% air/5% CO2 atmosphere for 72 h. After incubation, cells were labelled with anti‐CD4 and 20 000 cells were acquired by flow cytometry. Representative dot plots of CFSE‐labelled cells representing chitosan‐induced inhibition of ConA‐induced mitogenesis are shown. Percentage of daughter cells in the box is included. (a) Unstimulated control and ConA stimulated cells; (b) left: ConA + chitosan; middle: ConA for 24 h and then chitosan for further 72 h; right: chitosan for 24 h and then ConA for further 72 h.

Cona Polysaccharide, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cona+polysaccharide/pmc06495552-109-11-35?v=CH+Instruments
Average 90 stars, based on 1 article reviews

cona polysaccharide - by Bioz Stars, 2026-07

90/100 stars

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1) Product Images from "Ability of the polysaccharide chitosan to inhibit proliferation of CD4+ lymphocytes from mucosal inductive sites, in vitro and in vivo"

Article Title: Ability of the polysaccharide chitosan to inhibit proliferation of CD4+ lymphocytes from mucosal inductive sites, in vitro and in vivo

Journal: Cell Proliferation

doi: 10.1111/j.1365-2184.2009.00634.x

Effect of chitosan on ConA‐induced T‐cell proliferation in vitro. Mesenteric lymph node (MLN) lymphocytes from normal rats labelled with succinimidyl ester of carboxyfluorescein diacetate (CFSE) were stimulated with 2.5 μg/ml of mitogen in presence or absence of chitosan (1–100 μg/ml) at 37 °C in RPMI 1640 medium supplemented with 10% foetal calf serum in a 95% air/5% CO2 atmosphere for 72 h. After incubation, cells were labelled with anti‐CD4 and 20 000 cells were acquired by flow cytometry. Representative dot plots of CFSE‐labelled cells representing chitosan‐induced inhibition of ConA‐induced mitogenesis are shown. Percentage of daughter cells in the box is included. (a) Unstimulated control and ConA stimulated cells; (b) left: ConA + chitosan; middle: ConA for 24 h and then chitosan for further 72 h; right: chitosan for 24 h and then ConA for further 72 h.

Figure Legend Snippet: Effect of chitosan on ConA‐induced T‐cell proliferation in vitro. Mesenteric lymph node (MLN) lymphocytes from normal rats labelled with succinimidyl ester of carboxyfluorescein diacetate (CFSE) were stimulated with 2.5 μg/ml of mitogen in presence or absence of chitosan (1–100 μg/ml) at 37 °C in RPMI 1640 medium supplemented with 10% foetal calf serum in a 95% air/5% CO2 atmosphere for 72 h. After incubation, cells were labelled with anti‐CD4 and 20 000 cells were acquired by flow cytometry. Representative dot plots of CFSE‐labelled cells representing chitosan‐induced inhibition of ConA‐induced mitogenesis are shown. Percentage of daughter cells in the box is included. (a) Unstimulated control and ConA stimulated cells; (b) left: ConA + chitosan; middle: ConA for 24 h and then chitosan for further 72 h; right: chitosan for 24 h and then ConA for further 72 h.

Techniques Used: In Vitro, Incubation, Flow Cytometry, Inhibition, Control

Expression of markers CD25, CD71 and CD95L in lymphocytes stimulated with ConA and chitosan. Mesenteric lymph nodes (MLN) cells (1 × 106 cells/well) cultured for 72 h as described in Fig. 1 were harvested and stained with fluorochrome‐conjugated antibodies against CD4, CD71, CD25 and CD95L as described in the Materials and methods section. (A) Representative dot plots of CD25 and CD71 expression in MLN CD4+ cell cultures with (a) medium (basal); (b) chitosan; (c) ConA; (d) ConA 24 together with chitosan; (e) ConA 24 h and then chitosan; (f) chitosan 24 h and then ConA. (B) Mean of CD25 and CD71expression of 6–8 wells per experimental condition from two similar experiments. (C) Representative histograms of CD95L expression in the same experimental conditions of (A). (D) Mean of CD95L expression of 6–8 wells per experimental condition from two similar experiments. *P < 0.05 vs. basal; #P < 0.05 vs. chitosan or ConA. Other comparisons are indicated with lines in the figure (B and D).

Figure Legend Snippet: Expression of markers CD25, CD71 and CD95L in lymphocytes stimulated with ConA and chitosan. Mesenteric lymph nodes (MLN) cells (1 × 106 cells/well) cultured for 72 h as described in Fig. 1 were harvested and stained with fluorochrome‐conjugated antibodies against CD4, CD71, CD25 and CD95L as described in the Materials and methods section. (A) Representative dot plots of CD25 and CD71 expression in MLN CD4+ cell cultures with (a) medium (basal); (b) chitosan; (c) ConA; (d) ConA 24 together with chitosan; (e) ConA 24 h and then chitosan; (f) chitosan 24 h and then ConA. (B) Mean of CD25 and CD71expression of 6–8 wells per experimental condition from two similar experiments. (C) Representative histograms of CD95L expression in the same experimental conditions of (A). (D) Mean of CD95L expression of 6–8 wells per experimental condition from two similar experiments. *P < 0.05 vs. basal; #P < 0.05 vs. chitosan or ConA. Other comparisons are indicated with lines in the figure (B and D).

Techniques Used: Expressing, Cell Culture, Staining

Effect of chitosan on ConA‐induced T‐cell proliferation ex vivo. Mesenteric lymph node (MLN) cells from rats fed a single (a) or five doses (b) of diluent (Dil) or of 1–5 mg chitosan (Ch1–5) were labelled with succinimidyl ester of carboxyfluorescein diacetate (CFSE) and stimulated with 2.5 μg/ml ConA as described in the Materials and methods section. After incubation, cells were labelled with anti‐CD4 and 20 000 cells were acquired by flow cytometry. (a) Representative dot plots of CFSE‐labelled cells representing chitosan‐induced inhibition of ConA‐induced mitogenesis are shown. The percentage of daughter cells in the box is included. Bars are means of data from 4–6 rats per treatment. In culture supernatants, the production of INF‐γ was evaluated by ELISA. (b) Representative dot plots of CFSE‐labelled cells representing inhibition of ConA‐induced mitogenesis after sustained administration of chitosan.

Figure Legend Snippet: Effect of chitosan on ConA‐induced T‐cell proliferation ex vivo. Mesenteric lymph node (MLN) cells from rats fed a single (a) or five doses (b) of diluent (Dil) or of 1–5 mg chitosan (Ch1–5) were labelled with succinimidyl ester of carboxyfluorescein diacetate (CFSE) and stimulated with 2.5 μg/ml ConA as described in the Materials and methods section. After incubation, cells were labelled with anti‐CD4 and 20 000 cells were acquired by flow cytometry. (a) Representative dot plots of CFSE‐labelled cells representing chitosan‐induced inhibition of ConA‐induced mitogenesis are shown. The percentage of daughter cells in the box is included. Bars are means of data from 4–6 rats per treatment. In culture supernatants, the production of INF‐γ was evaluated by ELISA. (b) Representative dot plots of CFSE‐labelled cells representing inhibition of ConA‐induced mitogenesis after sustained administration of chitosan.

Techniques Used: Ex Vivo, Incubation, Flow Cytometry, Inhibition, Enzyme-linked Immunosorbent Assay

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Journal: Cell Proliferation

Article Title: Ability of the polysaccharide chitosan to inhibit proliferation of CD4+ lymphocytes from mucosal inductive sites, in vitro and in vivo

doi: 10.1111/j.1365-2184.2009.00634.x

Figure Lengend Snippet: Effect of chitosan on ConA‐induced T‐cell proliferation in vitro. Mesenteric lymph node (MLN) lymphocytes from normal rats labelled with succinimidyl ester of carboxyfluorescein diacetate (CFSE) were stimulated with 2.5 μg/ml of mitogen in presence or absence of chitosan (1–100 μg/ml) at 37 °C in RPMI 1640 medium supplemented with 10% foetal calf serum in a 95% air/5% CO2 atmosphere for 72 h. After incubation, cells were labelled with anti‐CD4 and 20 000 cells were acquired by flow cytometry. Representative dot plots of CFSE‐labelled cells representing chitosan‐induced inhibition of ConA‐induced mitogenesis are shown. Percentage of daughter cells in the box is included. (a) Unstimulated control and ConA stimulated cells; (b) left: ConA + chitosan; middle: ConA for 24 h and then chitosan for further 72 h; right: chitosan for 24 h and then ConA for further 72 h.

Article Snippet: Compared to the basal condition, the increment was significant either with ConA or chitosan ( P < 0.05) ( ) and highest values were found in cultures with simultaneous addition of both stimuli (ConA + Chi) or pre‐treatment with ConA (ConA24 + Chi) ( P < 0.05).

Techniques: In Vitro, Incubation, Flow Cytometry, Inhibition, Control

Journal: Cell Proliferation

Article Title: Ability of the polysaccharide chitosan to inhibit proliferation of CD4+ lymphocytes from mucosal inductive sites, in vitro and in vivo

doi: 10.1111/j.1365-2184.2009.00634.x

Figure Lengend Snippet: Expression of markers CD25, CD71 and CD95L in lymphocytes stimulated with ConA and chitosan. Mesenteric lymph nodes (MLN) cells (1 × 106 cells/well) cultured for 72 h as described in Fig. 1 were harvested and stained with fluorochrome‐conjugated antibodies against CD4, CD71, CD25 and CD95L as described in the Materials and methods section. (A) Representative dot plots of CD25 and CD71 expression in MLN CD4+ cell cultures with (a) medium (basal); (b) chitosan; (c) ConA; (d) ConA 24 together with chitosan; (e) ConA 24 h and then chitosan; (f) chitosan 24 h and then ConA. (B) Mean of CD25 and CD71expression of 6–8 wells per experimental condition from two similar experiments. (C) Representative histograms of CD95L expression in the same experimental conditions of (A). (D) Mean of CD95L expression of 6–8 wells per experimental condition from two similar experiments. *P < 0.05 vs. basal; #P < 0.05 vs. chitosan or ConA. Other comparisons are indicated with lines in the figure (B and D).

Techniques: Expressing, Cell Culture, Staining

Journal: Cell Proliferation

Article Title: Ability of the polysaccharide chitosan to inhibit proliferation of CD4+ lymphocytes from mucosal inductive sites, in vitro and in vivo

doi: 10.1111/j.1365-2184.2009.00634.x

Figure Lengend Snippet: Effect of chitosan on ConA‐induced T‐cell proliferation ex vivo. Mesenteric lymph node (MLN) cells from rats fed a single (a) or five doses (b) of diluent (Dil) or of 1–5 mg chitosan (Ch1–5) were labelled with succinimidyl ester of carboxyfluorescein diacetate (CFSE) and stimulated with 2.5 μg/ml ConA as described in the Materials and methods section. After incubation, cells were labelled with anti‐CD4 and 20 000 cells were acquired by flow cytometry. (a) Representative dot plots of CFSE‐labelled cells representing chitosan‐induced inhibition of ConA‐induced mitogenesis are shown. The percentage of daughter cells in the box is included. Bars are means of data from 4–6 rats per treatment. In culture supernatants, the production of INF‐γ was evaluated by ELISA. (b) Representative dot plots of CFSE‐labelled cells representing inhibition of ConA‐induced mitogenesis after sustained administration of chitosan.

Techniques: Ex Vivo, Incubation, Flow Cytometry, Inhibition, Enzyme-linked Immunosorbent Assay

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